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" . . . the scanning tunneling microscope as well as the atomic force microscope. Today, these microscopes have an extremely high performance and a high enough resolution to display features on a molecular level. Such microscopes tend have one crucial drawback however, insofar as they are usually limited to viewing the surface of a specimen or to viewing a very thin layer. It is due to this fact that light microscopes have remained indispensable in biological and medical research. Not only do they provide three-dimensional images of live cells, they can also map out biochemical processes inside the cell."
"Fluorescence microscopy permits us to target as well as to mark specific sub-cellular components with dyes. After exposing them to light, they fluoresce, thereby disclosing their exact location to us."
"This advance for the first time allowed resolutions well below 50 nm- something almost inconceivable for a light microscope using conventional objectives and focused light. . . . Dyba and Hell's pioneering experiment shows for the first time that it is indeed possible to increase the focus into the double-digit nanometer range." ... [more]
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